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The pET-52b(+) MCS sequence was PCR amplified between the XbaI and BlpI restriction sites, adding a BglII restriction site at the 5' extremity and a HindIII restriction site at the 3' extremity

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Phytomedicine 101 , 154102 (2022)

Global and deep molecular analysis of microbiota signatures in fecal samples from patients with irritable bowel syndrome