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Metabolomics has been defined as the measurement of the metabolite pool that exists within a cell under a particular set of conditions (Fiehn 2002), while metabonomics was earlier defined as the quantitative measurement of the dynamic multiparametric metabolic response of living systems to pathophysiological stimuli or genetic modification (Nicholson et al

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The cells were then fixed with 1% osmium tetroxide for 2 h at 37 C