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Absorbance at 410 nm was measured once every minute at 7 time points using Microplate Spectrophotometer PowerWave XS(BioTek Instruments, Winooski, VT, USA)

Moreover, SuperScript IV (SSIV) showed no significant change in any mutation types compared to TGIRT (Supplementary Fig

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Smaller mixing volumes result in higher levels, allowing for smaller injection volumes but possibly shorter shelf life