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Lipopolysaccharide: basic biochemistry, intracellular signaling, and physiological impacts in the gut

PLoS ONE 4 , e6313 (2009)

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385 Mito-TEMPO (MT), another mitochondrial superoxide scavenger, also prevents muscle atrophy via alleviating mitochondrial dysfunction, oxidative stress and inflammatory

Introduction The global water cycle faces unprecedented anthropogenic stress, with aquatic ecosystems increasingly serving as the ultimate sinks for a vast array of contaminants originating from the technosphere.14 The relentless expansion of industrialisation and intensive agriculture discharges a complex mixture of chemical stressorsranging from heavy metal cations (e.g., Cd2+, Hg2+, Pb2+) to bioactive micropollutantsposing a profound threat to ecological stability.57 Legacy water treatment infrastructures are fundamentally ill-equipped to eliminate these recalcitrant contaminants, which often persist at trace concentrations (ng L1 to g L1) yet exert potent biological effects

Pull down assay for GPX4 PC12 cells were treated with DAQ probe (DA-yne probe contains FeCl 3 solution) or DMSO for 6 hours, and then were harvested and lysed with RIPA buffer supplemented with protease inhibitor cocktails and phosphatase inhibitor cocktails