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The soluble clarified fraction was recovered by centrifuging at 75,000 g for 20 min at 4 C and applied to a Superdex 200 26/600 column (Cytiva) for SEC, which was run as above

The development of a highly sensitive high-pressure liquid chromatography with electrochemical detection (HPLCEC) adduct assay (Muldrew et al

Overexpression of human hepsin in adult mice with AAV administration For the APAP susceptibility test, mice were made to overexpress human hepsin in the liver through transduction with 2.5 10 10 viral genome (vg) per mouse of AAV-hHPN WT /hHPN RS /EGFP via retro-orbital injection

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B-cell epitope prediction was performed using the Immune Database analysis resource antibody epitope prediction tool 80 with a threshold of 0.5