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The GSH-containing syringe of the stopped-flow spectrofluorometer was supplemented again with 0.4 U/mL GR and 0.25 mM NADPH to remove trace amounts of GSSG and to prevent the thermodynamically favored reverse reaction 32

Peptide-spectra matches were then grouped based on protein ID, excluding peptides with summed reporter ion intensities for the WT channels 0.5, non-unique or non-tryptic peptide sequences

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Sequential regulation of ferroportin expression after erythrophagocytosis in murine macrophages: early mRNA induction by haem, followed by iron-dependent protein expression

Glutathione affinity purification is commonly used to isolate and purify glutathione S-transferase (GST)-tagged fusion proteins from total cellular proteins in lysates