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Analytes were determined by atomic absorption spectroscopy (AAS-Perkin Elmer) in all the study matrices, and the analytical method used was validated using certified reference material for soil (SO-3, Canadian Certified Reference Material Project , Canada), leaves (NIST-1547), and animal samples (NIST-1577b, bovine liver ), with recovery rates over 79%

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Melatonin affects the T cell subpopulation in gestation by increasing the differentiation of Th17 and Tregs (94, 95)

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The FDA based its expansion for all stages of liver disease on the data from a Phase I trial that showed no new safety signals

ELISA assay After euthanizing the mice, the plasma samples collected from experimental animals blood were obtained by centrifugation at 3000 rpm for 15 min