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The mitochondrial superoxide that was detected using MitoSox reagent was observed through a fluorescence microscope at an excitation/emission of 510/580 nm (red color)

The cells were incubated with the indicated reagents for further experiments after 48 h of transfection

Nevertheless, the clinical performance of non-invasive vaccines is often restricted by their low bioavailability, primarily due to biological barriers that limit antigen absorption and stability in vivo (Grassly et al

J Med.Toxicol

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