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American Medical Association American Gastroenterological Association American Association For The Study of Liver Diseases Lafaine Grant, M.D

Ruiz-Alcaraz, Carlos M

The study showed that activation of PPAR stimulated mitochondrial biogenesis and suppressed ROS production, protecting the brain from mitochondrial and oxidative damage (90)

Abbreviations in the Examples are as follows: mM = millimolar uM = micromolar uL = micro liter GAP = glutamate and cystine and glycine mix GAP 5 = 4 uM sodium pyroglutamic acid, 5 uM cystine, 16 uM glycine GAP 10 = 8 uM sodium pyroglutamic acid, 10 uM cystine, 32 uM glycine GAP 20 = 16 uM sodium pyroglutamic acid, 20 uM cystine, 64 uM glycine NaPCA = sodium pyroglutamic acid (also known as sodium pyrrolidonecarboxylic acid) HR=4-hexylresorcinol BR=4-butylresorcinol IPR = 4-isopropylresorcinol ER = 4-ethylresorcinol PER = 4- (1-phenylethyl) resorcinol MR = 4-methylresorcinol 4-CHMR = 4- (cyclohexylmethyl) resorcinol DEC = Diethyl L-cystinate dihydrochloride CDME = L-Cystine dimethyl ester dihydrochloride DTBC = Ditert-butyl L-cystinate dihydrochloride Example 1 Example 1 evaluated various combinations of 4-alkyl resorcinols and amino acids constituting GSH building blocks for potentiating intracellular GSH production

Most of the commonly used oxidoreductase assays for glutaredoxins are either discontinuous and labor-intensive or unidirectional and coupled to the glutathione reductase-dependent consumption of NADPH 1,2,6,8,16,17,18,30

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