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The homozygous null mutation in mice is somewhat more severe than the dominant-negative effect in humans because the loss of collagen III protein is complete in the mouse

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This matters because even a small impurity or inconsistency in formulation can affect how the peptide works in the body and may increase the risk of unwanted side effects

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In a recent study Sheikh Abdur Rashid et al., prepared methotrexate-loaded almond oil-based nanoemulsion formulation by applying high shear homogenization technique for PsO treatment