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In vivo model included diverse organisms such as the nematode Caenorhabditis elegans ( Arabidopsis thaliana leaf infiltration model ( Galleria mellonella larvae ( Artemia salina ( In vitro studies using human cell lines have been extensively used to study pyocyanin, including NCI-H292 pulmonary carcinoma cells, 16HBE and NHBE (human bronchial epithelial cells), and A549 lung carcinoma epithelial cells (47, 48)

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In addition, decreased the expression of p-GSK-3 was also reported during glutamate-induced neurotoxicity 24

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The pill has also been shown to strip the body of zinc so supplementation is often required post-pill use