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This suggests a very good safety and tolerability profile indistinguishable from placebo in those trial settings

5-Amino-1MQ binds the NNMT active site with high selectivity and is widely used in published research as a chemical probe for NNMT function

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The reduced enzymatic activity increases cellular concentrations of both substrates that NNMT would otherwise consume: S-adenosyl-L-methionine (the universal cellular methyl donor) and nicotinamide (the NAD + salvage precursor)