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Western blot was performed using a polyclonal cleaved caspase-3 antibody (Asp-175 from Cell Signaling) and a corresponding secondary horseradish peroxidase linked polyclonal anti-rabbit antibody (Acris)

10.1007/s00726-016-2232-1 13 FelsensteinJ

Calcium mobilization assay Calcium mobilization assay to measure GHSR signaling was carried out in HEK-293T cells cultured in DMEM supplemented with 10% (vol/vol) FBS (Fisher Scientific)

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Smythies JR, Halsey JH

Coenzyme Q10 enhances mitochondrial electron transport efficiency and indirectly activates AMPK signaling, thus maintaining energy homeostasis and preventing mitochondrial depolarization (88)