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Samples were resuspended in 160 L EPPS buffer (200 mM, pH 8.0) using a probe sonicator (1 1015 pulses, power output 20%) and then digested with LysC (4 L of 0.5 g/L per sample, resuspended in HPLC grade water, Wako-chemicals, Fujifilm #125-05061) for 2 h at 37 C in a shaker incubator

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Ventilation frequency was maintained at 110 breaths/min, with tidal volume between 125 and 150 l

Maintain a Healthy Diet A diet rich in antioxidants can help support your bodys natural glutathione production and protect against oxidative stress