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Qualified samples were subjected to overnight hybridization reaction at 65C, where 5 l of total RNA samples were combined with 3 l of nCounter reporter CodeSet in 5 l of hybridization buffer and 2 l of nCounter capture ProbeSet for a total reaction volume of 15 l and incubated for 20 h

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doi: 10.3390/molecules30030652 236 CaputoMBonaELeoneISamMNuzzoAFerreroAet al

The neutralization efficiency of the preservative system in the test sample was determined for the neutralizer: Eugon LT 100 at a sample dilution of 10 1