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Quantitation of peptides and LCMS/MS analysis were performed by CPOS

There are three GPX4 isoforms: cytosolic (cGPX4), mitochondrial (mGPX4), and nuclear (nGPX4), with distinct spatiotemporal expression patterns during embryonic development and adult life

Research categories and tags for this page include Mitochondria, Cellular Research

This advanced protocol is not for beginners and requires careful monitoring and medical supervision

Grading of recommendation as per OCEBM levels of evidence (March 2009)

2.4 Muscle proteome Frozen gastrocnemius tissue from the right hindlimb was crushed with a pestle and placed in homogenization buffer (1% Triton X-100, 50 mM HEPES, 12 mM Na pyrophosphate, 100 mM NaF, 10 mM EDTA, protease inhibitor, phosphatase inhibitor) with a stainless-steel bead