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Cell debris was removed by centrifugation (20 min, 4 C, 15,000 g ), and the protein concentration of the supernatants was determined using the Pierce BCA (Thermo Fisher Scientific) with a bovine serum albumin standard (yielding an average of 5.8 g/L protein)

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Ferroptosis in cancer: revealing the multifaceted functions of mitochondria

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Genetic susceptibility plays a role, with HLA-Cw6 and CARD14 gene mutations increasing the risk

In both clinical research and wellness settings, dosing is relatively consistent low microgram amounts designed for daily use