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Tissue was homogenized by probe sonication (2 10 pulses, 40% power output), and particulate matter was removed by passing samples through 0.4 m syringe filters

Resilience of Portulaca plants to environmental stresses and the economic potential of their bioactive compounds

These moderated t-tests in DESeq2 are shrinkage-based two-sided Wald tests (H 0 : log 2 FC = 0), which incorporate empirical Bayes moderation to improve dispersion estimation across genes, allowing for more reliable inferences 85

doi: 10.1016/S0140-6736(13)62227-8 180 ClinicalTrials.gov

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