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After 30 min of blocking with blocking buffer (Dulbeccos PBS containing 0.2% bovine serum albumin, pH 7.4), at RT, the plates were washed 3x with wash buffer (Dulbeccos PBS containing 0.05% Tween 20, pH 7.4)

After separating from the ventral ramus, the nerve travels medially before joining a branch from the ramus gray to contribute sympathetic fibers, then passes through the intervertebral foramen and osteofibrous foramen near the pedicle, reentering the spinal canal

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