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To view a copy of this licence, visit About this article Cite this article Monteiro, J.P.P., dos Santos, C.C.M., de Queiroz, J.P.M

doi: 10.1016/j.ucl.2005.08.007 63 BurnettALMusickiB

Briefly, the thermocycling protocol followed a pre-incubation at 95C for 10 minutes, followed by 45 cycles of 3-Step amplification: 1) denature at 95C for 20 seconds