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Pharmacological interventions to ameliorate neuropathological symptoms in a mouse model of Lafora Disease

Serrano added that older populations were also at higher risk, as cases of B12 deficiency increase with age

Immunofluorescence staining of cultured neurons To determine the effect of lithium on neuronal morphology, neuron cultures were fixed with 4% paraformaldehyde for 30 min at room temperature, permeabilized with 0.25% Triton X-100 in phosphate buffer saline for 10 min, blocked with 5% donkey serum (Jackson Laboratories, PA) for 1 h at room temperature, and then incubated for 16 h at 4C with rabbit anti-microtubule-associated protein (MAP)2 antibody (Millipore, MA)

Around 200 cells from at least 6 images were quantified for each experimental group

10.3390/molecules190812280 212 VriensK.CoolsT

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