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UFL1 and DDRGK1 cDNAs were individually cloned into expression vectors, including pLenti CMV GFP Hygro Vector (Addgene, #17446), pFC14K HaloTag CMV Vector (Promega, EU113047), pFN21A HaloTag CMV Vector (Promega, EU621374), pFC32K Nluc CMV-neo Flexi Vector (Promega, KF811456), pFN31K Nluc CMV-neo Flexi Vector (Promega, KF793053), pCW57.1 plasmid backbone (Addgene, #41393), and pET28a plasmid backbone, using the In-Fusion cloning system (HiFi DNA Assembly Master Mix, NEB #E2621)

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therefore, vitamin D supplementation in an IMID combined test could further improve the therapeutic effect of anti-CD38 antibodies ( CD38 mRNA levels in MM cells and the amount of CD38 on the cell surface