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As we age, glutathione levels in the body gradually decrease, which can lead to increased oxidative stress and premature aging

However, the assay has the disadvantage that in comparison with the fluorescent spot test and the spectrophotometric assay, it is a time-consuming and technically difficult test

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[44] LA is able to scavenge reactive oxygen and reactive nitrogen species in a biochemical assay due to long incubation times, but there is little evidence this occurs within a cell or that radical scavenging contributes to the primary mechanisms of action of LA

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