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MCF10A cells were obtained from American Type Culture Collection (Manassas, VA, United States), and maintained in DMEM/F12 (Nakarai Tesque Inc., Kyoto, Japan) supplemented with 20 ng/mL of epidermal growth factor (EGF) (Peptide Institute Inc., Osaka, Japan), 100 ng/mL of cholera toxin (Sigma, St

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four single-cell RNA sequencing libraries) to reconstruct CD4 T cell subtype dynamics, pseudotime trajectories, and ligandreceptor (LR) interaction networks

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