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Molecular Docking: docking experiments were performed using Glide included in the Schrodinger Package, Maestro interface

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differing normalization and batch effects) and possess sufficient corresponding clinical data (particularly long-term follow-up and survival status) to perform a reliable external validation of the prognostic risk score

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The membrane was washed three times in PBS and incubated with the corresponding horseradish peroxidase (HRP)-conjugated secondary antibody (Amersham International) for 1 h