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All isoforms bound seven Zn(II) ions, and their identities were confirmed by mass spectrometry (Table S3)

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therefore, the cells were treated for two weeks prior the experiments with 25 g/ml Plasmocin (Invitrogen, Sweden)

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Thus, antagonists cause a prompt inhibition of the release of gonadotropins and sex steroids in distinction to the LH-RH agonists