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Where indicated, the protein synthesis inhibitor cycloheximide (CHX) (Sigma-Aldrich) and the proteasome inhibitor MG132 (Sigma-Aldrich) were added to HEK293 cells cultured with a final concentration of 25 g/ml and 10 M respectively in a time-course experiment

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doi: 10.1016/j.cell.2016.10.043

When applied in the condition of the already established severe acute pancreatitis, an obvious salutary effect was consistently noted

In one example of any of I1-I29 (I38), the pharmaceutical composition does not include a tris base

For the preparation of nuclear extracts, frozen liver pieces were homogenized in homogenization buffer (20 mM HEPES, 70 mM sucrose, 220 mM Mannitol, 2 mM EDTA, 0.5 mg/ml BSA, 0.1 mM PMSF, 1.0 mM DTT, pH 7.5)