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Together, these observations indicate sustained perturbations in immune homeostasis well after viral clearance

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This fragment was digested with EcoRI (NEB) and ligated into pGex-6p1 (GE Healthcare) prepared by digestion with BamHI and blunting with Mung Bean Nuclease (NEB) to generate pJB-GST-HTS

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Transient transfections were performed using 25 kDa linear polyethylenimine (PEI) (purchased from Polysciences Inc

Additionally, PQ downregulates SLC7A11 and GPX4 expression and upregulates cyclooxygenase-2 expression, thereby promoting oxidative stress and ferroptotic cell death ( 4.2 Genetic models 4.2.1 SNCA models Overexpression of wild-type or mutant (A53T, A30P) -syn induces Lewy body-like inclusions in dopaminergic neurons, leading to significant neuron damage and degeneration