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10.1080/00021369.1969.10859329 28 KeldermanC

Furthermore, although our secondary analyses supported that the influence of PO is independent of which progenitor presents obesity, in line with growing scientific evidence [3, 7], we cannot discard that, at least in part, this lack of observed differences could be due to limited statistical power (i.e., reduced sample size, unequal number of subjects within groups)

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Molecular analyses via Western blotting and reverse transcription quantitative polymerase chain reaction (RT-qPCR) revealed that Cd exposure led to upregulation of mesenchymal markers (ZEB1, N-cadherin, vimentin) and downregulation of the epithelial marker E-cadherin, confirming the induction of EMT (51)