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1 mL of DPPH solution was added to a small volume ( 5 L) each test compound dissolved in DMSO

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The growth medium was replaced every 7 days, and experiments were performed after 14 DIV

When Progress Stalls The shape of the stall is more informative than an automatic dose increase

In addition, proteasome inhibition, which reduces NF-B activation, has been shown to enhance cellular antioxidant defenses via an Nrf2-dependent transcriptional mechanism, suggesting the indirect effects of NF-B on antioxidant regulation (Karin and Ben-Neriah, 2000

Thus, microglia and astrocytes play key roles in the pathogenesis and progression of cerebral ischemia