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After the GST-tag was cleaved by PreScission protease (GE Healthcare), the proteins were further purified by a second glutathione Sephararose 4B column, the collected fractions containing target proteins were then loaded into MonoQ column for further purification with a linear gradient elution, and fractions containing VanR or mutant VanR from MonoQ were finally purified through Supperdex 75 (GE Healthcare) column equilibrated with buffer (20 mM Tris, pH 8.0, 200 mM NaCl, 2 mM DTT)

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In view of robust patient blood management practices and despite stringent selection criteria, only ~ 56% of enrolled subjects were transfused (site range 1882%)

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Ginkgo Biloba Leaf Extract (EGb 761) and Its Specific Acylated Flavonol Constituents Increase Dopamine and Acetylcholine Levels in the Rat Medial Prefrontal Cortex: Possible Implications for the Cognitive Enhancing Properties of EGb 761. International Psychogeriatrics, vol