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Kolodziejczyk et al., 2019

Importantly, secondary FAD depletion has been observed in diseases without primary flavoprotein mutationssuch as L-2-hydroxyglutaric aciduria and Friedreichs ataxia

The MoClo Toolkit (Weber et al., 2011) to assemble sgRNAs constructs and Golden Gate cloning ( The Golden Gate cloning strategy is a very fast and flexible assembly for CRISPR/Cas construct designing (Tran et al., 2021