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Best Practices After Reconstitution: Refrigerate immediately after reconstitution Avoid unnecessary agitation Protect solutions from excessive light exposure Minimize repeated temperature fluctuations Maintain detailed laboratory records Follow established handling procedures Important: If cloudiness, discoloration, or visible particulate matter appear, researchers should review laboratory protocols and handling procedures before continuing use

This is shown in the following illustration, and since hexane is less dense than water, the hexane phase floats on the water phase

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Some feel better within 2-3 weeks, others need several months

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[PMC free article: PMC4110209] [PubMed: 25067957] 229