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All samples (FLAG-PRMT1-binding protein) were analyzed on an UltiMate 3000 RSLCnano system coupled online with Q Exactive HF mass spectrometer through a Nanospray Flex ion source (Thermo Fisher Scientific)

While simulating the droplet trajectories, we assumed typical solid-cone injections and tracked the transport for 1-mg spray shot while comparing the TSPD trends from the CFD predictions with the corresponding experimental drug delivery patterns

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Lasalvia et al., 2016)

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