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While OXPHOS is the primary pathway for energy generation in static T cells (45), activated T cells require the GLUT1 receptor to increase glucose uptake and promote the upregulation of glycolytic enzymes to catalyse glycolysis, resulting in increased pyruvic acid production (47)

Sci Rep 8 , 10438 (2018)

Hasegawa H, Suzuki K, Suzuki K, Nakaji S, Sugawara K

Prolonged Glucagon Infusion Does Not Affect Energy Expenditure in Individuals with Overweight/Obesity: A Randomized Trial

Bind or No Bind Pull down assays offer a binary "yes" or "no" answer regarding any possible interaction between two proteins, thus establishing this technique as essential for the study of cellular pathways via protein-protein interactions