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After the TUNEL method, the sections were stained with DAPI (4,6-diamidino-2-phenylindole) and mounted with Fluoromount (SouthernBiotech, Birmingham, AL, USA), and FITC-labeled apoptotic cells were then imaged on an Olympus BX5 fluorescence microscope imaging system (Olympus America, Melville, NY, USA)

doi: 10.1681/asn.2015020196, 154

Lee J, Lee H, Goh U, Kim J, Jeong M, Lee J, Park J-H

Restriction enzyme cut sites used for homologous overlaps for Gibson Assembly have been provided

As these movements continue, creases become more visible

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