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MTX-induced hepato-renal damage was assessed by serum toxicity biomarkers (AST, ALT, BUN, and creatinine), oxidative stress estimation (MDA, GSH, SOD, CAT, peroxidase, and glutathione reductase), interleukins profiling (TNF-, IL-1, IL-6, and IL-12), tissue histopathology and immunohistochemical (caspase-3 and NFkB) examination

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The authors showed that co-administration of curcumin with Al 3+ repressed the activities of phosphorylase kinase, protamine, PKC and AChE, while superoxide dismutase, glutathione peroxidase, glutathione- S -transferases and Na + , K + -ATPase levels were significantly elevated [308]