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In the granulosa cells of patients with PCOS and in letrozole-induced rat models, the HIF-1/BNIP3 mediated mitophagy is suppressed, resulting in a decrease in the clearance capacity of damaged mitochondria and accumulation of ROS, thereby inducing mitochondrial dysfunction [71]

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Construction of yeast expression vectors The P1 coding sequences for PV1 wildtype strain Mahoney (Gene Bank: KU866422.1) and the Mahoney-SC7 mutant were optimized for yeast expression and synthesized by Genscript (Nanjing, China)

Originally identified during investigations involving sleep physiology and neurological regulation, DSIP peptide has attracted substantial scientific interest due to its unique structure and potential interactions with biological systems associated with sleep cycles and neuroendocrine signaling