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The cell culture supernatant (containing detached apoptotic cells) and the adherent cells were harvested by trypsinization and centrifuged at 500 g for 5 min, before the resulting pellets were washed in staining media (PBS, 0.5% FBS)

doi:10.1016/j.jconrel.2021.12.034 119

Ethics approval The study was approved by the Institutional Review Board of the National Cancer Center Research Institute (2015-159)

Each of these components has unique properties that contribute to a balanced nutrient profile

Electron transfer in mitochondria and chloroplasts for energy metabolism and photosynthesis is one of the major topics

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