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The GST protein is fused to 20 amino acid His-Tag at N-terminus

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Brgel, J

nucleic acid sequences coding for the desired molecules can be obtained using recombinant methods known in the art, such as, for example by screening libraries from cells expressing the gene, by deriving the gene from a vector known to include the same, or by isolating directly from cells and tissues containing the same, using standard techniques

Thermo Fisher Scientific) connected to either an Orbitrap Fusion Lumos Tribrid or a Q Exactive HF-X mass spectrometer (both from Thermo Fisher Scientific)