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The -opioid receptor agonist U50448H provides another example, as it inhibits NLRP3/ caspase-1-induced pyroptosis in this case by activating cholinergic anti-inflammatory pathways and promoting the polarization of alveolar macrophages to the M2 type [131]

Handling and Reconstitution Protocol This product is supplied as a lyophilized powder and requires reconstitution with bacteriostatic water for laboratory use

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The treatment typically takes about 30-60 minutes with some exceptions

Subsequently, the sections were permeabilized with a 0.5% Triton X-100 (Sigma-Aldrich, United States) PBS solution for 15 min