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At the end of the experiment, the cells were washed once with Hanks balanced salt solution (HBSS) and cell viability was assessed by adding 20 L of the CellTiter 96 AQueous One Solution reagent (Promega, Fitchburg, WI) to 100 l HBSS, followed by incubation for 60 min at 37 C, and the absorbance was measured at 490 nm using the Infinite 200 PRO plate-reader (Tekan, Switzerland)

Wan et al., 2025)

Vessel diameters were measured at the 80 mmHg control pressure in Ca 2+ -free PSS to assess the level of active resting tone in the artery

doi: 10.3390/jcm9123941 251 AzpirozFDubrayCBernalier-DonadilleACardotJMAccarinoASerraJet al

Even, adverse effects are very scarce which are reported above

In summary, the activation of microglia and astrocytes, and the subsequent release of pro-inflammatory cytokines and ROS, create an unfavorable environment for neuronal damage and death