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Drug-metabolising enzymes (DMEs) play an important role in scavenging the waste products of lipid metabolism and oxidative metabolism and maintaining homeostasis of liver 4,5 , consisted of groups of enzymes, such as glutathione S-transferases (GSTs)

It also has been linked to influencing other growth pathways, like increasing gene expression for repair and even modulating neurotransmitter systems (dopamine, serotonin, etc., in brain injury models)

The following sgRNAs for GCLC CRISPRCas9 KO were used: sgNTC forward: 5-CACCGGAGGCTAAGCGTCGCAA-3

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For a bioproject-based analysis, reads were pooled, the unique SFB-positive sequences for each bioproject were identified, and their abundance calculated using the R packages Biostrings v2.68.1 and SeqinR v4.2.30