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Chromatographic peak areas and retention times were analyzed using Multiquant 3.0.2 software, and metabolites were identified by matching retention times with reference standards

The positioning of the 296 phenol suggests that in the absence of NADPH it orients to block access to the isoalloxazine N5 and prevent non-specific reduction or reoxidation of the flavin, kinetically restricting promiscuous/futile electron transfer out of the TGR population such that even low reduction potential non-native reductants fail to reduce the FAD significantly on timeframes relative to catalysis

doi: 10.1016/j.tox.2020.152665 100 HouJLeiZCuiLHouYYangLAnRet al

The extent of publication bias was assessed using the funnel plot and Eggers regression test