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Horseradish peroxidase-conjugated secondary antibodies (Supplementary Table 6) were detected by ECL using ECL SuperSignal West Pico or Femto (34087 or 34096, Pierce) followed by exposure on autoradiography film

Restivo and her staff

That distinction matters clinically because it preserves the feedback loop that governs GH secretion, reducing the risk of the side effects associated with exogenous HGH administration at therapeutic doses

It prevents cellular oxidation and inhibits cell-free radical damage [24,25,26]

This study used non-invasive collagen density measurement (optical profilometry), not biopsy

doi: 10.22092/ARI.2022.358619.2269 80 ZahranFMohamadAZeinN