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The cell resuspensions were then incubated at room temperature (~22 C) for 1 h

For passaging of organoids, the collagen domes were incubated with 1 mg ml 1 collagenase from Clostridium histolyticum (Sigma-Aldrich) for 30 min at 37 C, and digestion monitored using bright-field microscopy (small clumps of cells)

2020 [doi: 10.1089/ars.2019.7977] Martin-Hidalgo D, Bragado MJ, Batista AR, Oliveira PF, Alves MG

N.MaB.ZellaD.FamootoA.RavelJ.AdebamowoC

Glucocorticoids and insulin regulate expression of the human gene for insulin-like growth factor-binding protein-1 through proximal promoter elements