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The validation of the supervised models involved calculating the area under the curve and assessing the error rate through Balanced Error Rate (BER) and overall error (OVERALL) metrics computed on the first and second components using maximum, centroid, and Mahalanobis distance 45

However, the decrease is more pronounced at 37C than 27C, dropping within one while progressively decreasing after 2 hours at 27C (Figure 2)

Notably, these types of studies are limited by subjective measures of ascorbic acid exposure, which may be even less reliable in cognitively impaired study participants

The microplate was then incubated at 22 C for 10 minutes