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For plasmid transformation into yeast cells, a standard lithium acetate-based approach was used as described above

Immunity (2013) 38(3):596605.10.1016/j.immuni.2012.11.020 78 PhanTGPausDChanTDTurnerMLNuttSLBastenAet alHigh affinity germinal center B cells are actively selected into the plasma cell compartment

By normalizing each target genes expression to the GADPH level, the relative quantification of its expression was carried out

The antibiotic nourseothricin was purchased from WERNER BioAgents (Jena, Germany)

Although there is no agreement on the optimal parameters, researchers studying nVNS have used parameters similar to those of the VNS analogues implanted in the neck in human clinical trials, and the following are some of the nVNS-specific stimulation parameters used in the experiments ( Table 2 )