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TGF-3-loaded graphene oxide - self-assembling peptide hybrid hydrogels as functional 3D scaffolds for the regeneration of the nucleus pulposus

In order to examine the bipartite binding of OD + CR and IID of VP35 to the L protein respectively, mVP35 OD + CR (residues 57207) and IID (residues 208329) were individually subcloned into modified pFastBac vectors (Thermo Fisher Scientific), each carrying an N-terminal His 6 -MBP tag