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The addition of proteins (albumin), oligosaccharides (sucrose), and polysaccharides (chitosan) at different concentrations or at different times in the preparation of SeNPs influences size, morphology, and stability in liquid dispersion [42]

The following reaction system was used: 1 ng pGEX4T-2 plasmid, 50 mM Tris-HCl (pH 7.8), 5 mM MgCl 2 , 10 mM 2-mercaptoethanol, 10 g/ml BSA, 1 ng forward primer FP1 and 1 ng reverse primer RP1, 20 M dNTP, 1 M dideoxynucleotides (ddNTP), 2 units DNA Polymerase I (Invitrogen), and ddH 2 O to the reaction volume of 100 l

doi: 10.3892/ijo.2018.4614 223 XiongHNiZHeJJiangSLiXGongWet al

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The formula includes 1 gram of N-Acetyl-L-Cysteine (NAC) , which serves as a highly bioavailable precursor to glutathione

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